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KMID : 1161420220250080818
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Journal of Medicinal Food
2022 Volume.25 No. 8 p.818 ~ p.827
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Anti-Melanogenesis Activity of Crocodile (Crocodylus siamensis) White Blood Cell Extract on Ultraviolet B-Irradiated Melanocytes
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Joompang Anupong
Anwised Preeyanan
Luangpraditkun Kunlathida
Jangpromma Nisachon
Viyoch Jarupa
Viennet Celine
Klaynongsruang Sompong
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Abstract
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Ultraviolet (UV) radiation generates a range of biological effects in the skin, which includes premature skin aging, hyperpigmentation, and cancer. Therefore, the development of new effective agents for UV-related skin damage remains a challenge in the pharmaceutical industry. This study aims to test the inhibitory effect of crocodile white blood cell (cWBC) extract, a rich source of bioactive peptides, on ultraviolet B (UVB)-induced melanocyte pigmentation. The results showed that cWBC (6.25?400?μg/mL) could inhibit tyrosinase without adduct formation by 12.97?±?4.20% on average. cWBC pretreatment (25?100?μg/mL) had no cytotoxicity and reduced intracellular melanin to 111.17?±?5.20% compared with 124.87?±?7.43 for UVB condition. The protective role of cWBC pretreatment against UVB was exhibited by the promotion of cell proliferation and the prevention of UVB-induced morphological change as observed from F actin staining. The decrease of microphthalmia-associated transcription factor expression levels after cWBC pretreatment might be a mechanism by which cWBC suppresses UVB-induced pigmentation. These results suggest that cWBC could be beneficial for the prevention of UVB-induced skin pigmentation.
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KEYWORD
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crocodile white blood cell extract, melanin, melanocyte, MITF, UV
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